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Assisted Hatching Treatment in India

With assisted hatching, an embryologist uses micromanipulation under a microscope to create a small hole in the zona pellucida..The embryo is successfully implant into the uterine lining, an embryo hatches out of the zona pellucida and attach to the walls of the uterus.

Who should consider Assisted Hatching

Assisted Hatching has been suggested for infertile couples if:

  • The women is 38 years or older;
  • The woman has a high FSH level;
  • The woman has poor egg quality;
  • They have had poor embryo quality
  • They have failed to become pregnant in prior IVF cycles;
  • They are using frozen-thawed embryos in a Frozen Embryo Transfer (FET) cycle

Who should be treated with assisted hatching?

The most commonly used indications for assisted hatching with an in vitro fertilization case are:

3 Types of Assisted Hatching

Mechanical Assisted Hatching

As the name suggests, this method of assisted hatching involves manually grinding away a section of the zona pellucida. The aid of a micromanipulator machine is employed in this technique; where in the zona pellucida is carefully pierced using a fine glass needle. A pipette is then used to grind away a portion of the outer layer trapped by the glass needle

Chemical Assisted Hatching

The embryo is placed under a microscope in a petri dish containing culture fluid. A specific pipette and a hollow needle is used to hold the embryo in place. The hollow pipette contains an acidic solution, which is then released near the zona pellicuda. This acidic solution is meant to create a small hole in the zona pellucida by slowly digesting the outer layer. Chemical assisted hatching is one of the more common methods in use. This technique is also known as zona drilling

Laser-assisted Hatching 

It a small opening is made in the outer shell of the embryo, called the zona pellucida by laser.It  is an advance technology in IVF

Procedure for Assisted Hatching

This is a delicate procedurewhich  needs great skill and sophisticated instrumentation. Following are the steps:
1. By drilling with Acidified Tyrode’s solution
The embryo is kept in a dish containing culture solution where a pipette is used to hold the embryo. The embryologist takes a hollow needle containing an acidic solution, places it next to zona pellucid and release a small amount of the solution. This creates a small hole. After washing the embryo in culture media, it is transferred into the uterus solution and placed back in an incubator .
2. Mechanical opening by glass micro needle – Also known as partial zona dissection
3. Laser photoablation – Either a hole is made on the zona pellicuda or partial zona thinning is done using a laser. When this process is computer assisted, there is more precision and takes less time too.
Because of Assisted hatching, the rate of embryo implantation has improved.

Success rate of Assisted hatching

Alphine Life Solutions along with best hospitals for assisted hatching  in India have achieved 50%  success rate for Woman between the age of 35 to 39 years  at affordable cost
Intrauterine Insemination: Artificial Insemination for Infertility Treatment in India It  involves sperm being placed into the womb through a fine plastic tube. Sperm is collected and washed in a fluid. The sperm are passed through a tube that enters the cervix and extends into the womb.